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The presented protocol outlines a comprehensive assessment of the minimum inhibitory concentration mic and minimum bactericidal concentration mbc) values for bacterial cell cultures. Minimum bactericidal concentration (mbc) and minimum fungicidal concentration (mfc) assays determine the lowest concentration of an antimicrobial agent required to achieve bactericidal and fungicidal killing, defined as a 99.9% reduction in the initial inoculum. Methods for determining bactericidal activity of antimicrobial agents

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Approved guideline this document provides procedures for determining the lethal activity of antimicrobial agents. To do this, we used the minimum bactericidal concentration (mbc) determination approach to define bactericidal or bacteriostatic activity. This clsi guideline provides standardized laboratory methods for assessing bactericidal activity, ensuring accurate and reproducible testing for antimicrobial agents.

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The minimum bactericidal concentration (mbc) test can be used to assess the ability of an antimicrobial agent to kill a bacterial isolate

Mbc tests are performed after consultation in very select clinical situations when it is necessary to determine the bactericidal activity of an antimicrobial agent against a bacterial isolate. The mbc is determined using a series of steps, undertaken after a minimum inhibitory concentration (mic) test has been completed Also termed minimum lethal concentration (mlc), the mbc of an antibacterial agent is determined by subculturing the last clear mic tube onto the growth medium and examining for bacterial growth. This study aimed to optimize the treatment of ntm infection by selecting antibiotics with bactericidal activity for combination therapy